The coronavirus disease of 2019 (COVID-19) continues to pose challenges that require efficient methods, such as rapid and precise testing techniques, to limit its spread. Alternatively, a single combined O/N/S swab would improve the sensitivity in contrast to each site swabbed alone. It is concluded that immunofluorescent detection of SARS-CoV-2 antigen is a reliable method for rapid diagnosis under circumstances where at least two swabs, one nasal and one oropharyngeal, are analyzed. Finally, among multiple factors, only patient age revealed a significant negative association with antigenic detection in the saliva. Further, when considering positive detection in any of the O, N, and S samples, excellent agreements with rRT-PCR were achieved (Kappa = 0.91 and 0.97, respectively). The combined O/N/S swab provided the highest sensitivity (86% Kappa = 0.8), followed by nasal (76% Kappa = 0.68), whereas the saliva revealed the lowest sensitivity (66% kappa = 0.57). Each swab was analyzed using an immunofluorescent Quidel system. Fifty COVID-19 and twenty-six healthy patients were confirmed via rRT-PCR, and each patient was sampled via four swab methods: oropharyngeal (O), nasal (N), spit saliva (S), and combined O/N/S swabs. This study aimed to determine the level of agreement between SARS-CoV-2 fluorescent detection and a real-time reverse-transcriptase polymerase chain reaction (rRT-PCR), using different swab methods. However, there is no consensus on the best sampling method. Rapid antigen detection of SARS-CoV-2 has been widely used.
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